In this
blog, I will answer three questions about the topic “Bamboo in-vitro
cultivation”.
- Which are the main impediments for Bamboo multiplication?
There are a
few impediments that make the in vitro cultivation of Bamboo very interesting. Some
examples:
- There
are some species of bamboo that need to grow 100 years to product seeds (for
example Dendrocalamus asper) (Banerjee,
Gantait, & Pramanik, 2011)
- The
viability of seeds is short
- To
maintain a bamboo plantation, it is required to produce at least 100’000
seedlings per year (Venkatachalam,
Kalaiarasi, & Sreeramanan, 2015)
- Which measures can be taken to establish an efficient in vitro propagation system?
There are
different measures that can vary, depending on which cultivation step we are
looking at (for example Shoot bud development or Rooting of in vitro
regenerated shoots). Some examples are listed below:
- Media
variation: The use of different ingredients can increase the quantity of shoot
buds. For example, the use of 6-benzyl amino purine, kinetin, auxins, coconut
water and sucrose in different concentrations. Other ingredients can increase
the rooting. For example, the use of AgNO3 and indole-3-butyric acid
in the media.
- Seed
sterilization (to prevent contaminations).
- Growth
conditions. For example, the photoperiod.
- Days
of rooting before planting in the field (survival rate). (Venkatachalam
et al., 2015)
- Cocos milk or similar natural compounds seem to be effective ingredients for in vitro cultures. Are there any disadvantages too?
It seems
like coconut water has an essential effect on cell division. With its application,
the shoot bud multiplication is more rapid. A disadvantage of coconut water is,
that higher concentrations appear with an inhibitory effect, which often causes
vitrification of shoots. (Venkatachalam
et al., 2015)
Vitrification
is a well-known morphological and physiological disorder in in vitro
cultivation. It can cause big losses. The reduction of vitrification is an
important objective in in vitro cultivation. (Sharma
& Mohan, 2006)
Sources:
Banerjee, M., Gantait, S., &
Pramanik, B. R. (2011). A two step method for accelerated mass propagation of
Dendrocalamus asper and their evaluation in field. Physiology and Molecular
Biology of Plants, 17(4), 387–393.
http://doi.org/10.1007/s12298-011-0088-0
Sharma, U., & Mohan, J. S. S.
(2006). Reduction of vitrification in in vitro raised shoots of Chlorophytum
borivilianum Sant. & Fernand., a rare potent medicinal herb. Indian
Journal of Experimental Biology, 44(6), 499–505.
Venkatachalam, P.,
Kalaiarasi, K., & Sreeramanan, S. (2015). Influence of plant growth regulators
(PGRs) and various additives on in vitro plant propagation of Bambusa
arundinacea (Retz.) Wild: A recalcitrant bamboo species. Journal of Genetic
Engineering and Biotechnology, 13(2), 193–200.
http://doi.org/10.1016/j.jgeb.2015.09.006
